miapaca2 cells  (Addgene inc)

Journal: Journal of Sport and Health Science

Article Title: Exerkines: Potential regulators of ferroptosis

doi: 10.1016/j.jshs.2025.101032

Figure Lengend Snippet: Targets and regulatory effects of exerkines on ferroptosis in different types of cells.

Article Snippet: CTSB , STING1 , DNA damage , Inducing ferroptosis , PANC-1, MIAPaCa2 , PANC-1 cells injected subcutaneously into NOD-SCID mice , Kuang et al. .

Techniques: Injection, Membrane

Journal: bioRxiv

Article Title: Targeting de novo lipogenesis improves gemcitabine efficacy in pancreatic ductal adenocarcinoma

doi: 10.1101/2024.12.18.628624

Figure Lengend Snippet: Lipids were quantified from internal standards, and values are mean - scaled. C Representative confocal images of BOPIDY-stained Panc1 & MiaPaCa2 CON & GEMR cells lines, with ( D-E ) quantification of lipid droplets per cell and lipid droplet perimeter (µm). F Comparison of lipid droplet number and size between Panc1 and MiaPaCa2 cells. Statistical significance was determined by unpaired t test, * P < 0.05 ** P < 0.01. *** P < 0.001. CE cholesteryl ester, Cer ceramide, CL cardiolipin, DG diacylglycerol, LPC lysophosphatidylcholine, LPE lysophosphatidylethanolamine, PC phosphatidylcholine, PE phosphatidylethanolamine, PG phosphatidylglycerol, PS phosphatidylserine, SM sphingomyelin , TG triacylglycerol,

Article Snippet: Panc1 and MiaPaCa2 cells from ATCC were cultured in high glucose DMEM containing 4.5 g/L glucose and were supplemented with 10% FCS.

Techniques: Staining, Comparison

Journal: bioRxiv

Article Title: Targeting de novo lipogenesis improves gemcitabine efficacy in pancreatic ductal adenocarcinoma

doi: 10.1101/2024.12.18.628624

Figure Lengend Snippet: Canonical lipid synthesis pathways are shown in blue/black, while the alternative fatty acid desaturase 2 (FADS2)-mediated pathway is shown in red/orange. B De novo lipogenesis measured by tracing uniformly radiolabelled glucose into the lipid fraction of control (CON) and gemcitabine resistant (GEMR) Panc1 and MiaPaCa2 cells. Immunoblotting of key enzymes involved in de novo lipid synthesis in C Panc1 and D MiaPaCa2 CON and GEMR cell lines. E Analysis of AMP + -derivatised isomeric FA16:1 and FA 18:1 MUFA by ozone-enabled fatty acid discovery (OzFAD). F SCD1 activity measured by tracing uniformly labelled 13 C-palmitate (FA 16:0) into palmitoleic acid (FA 16:1 n- 7) using liquid chromatography-mass spectrometry (LC-MS). G immunoblotting of FADS2. H FADS2 activity measured by tracing uniformly labelled 13 C-palmitic acid into sapienic acid (FA 16:1 n- 10) by LC-MS. Unpaired t test, * P < 0.05, ** P < 0.01, *** P < 0.001. ELOVL elongation of very long chain fatty acids FADS2 fatty acid desaturase FAS fatty acid synthase 2 pACC phosphorylated acetyl-CoA carboxylase SCD1 stearoyl-CoA desaturase 1 tACC total ACC.

Article Snippet: Panc1 and MiaPaCa2 cells from ATCC were cultured in high glucose DMEM containing 4.5 g/L glucose and were supplemented with 10% FCS.

Techniques: Control, Western Blot, Activity Assay, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy

Journal: bioRxiv

Article Title: Targeting de novo lipogenesis improves gemcitabine efficacy in pancreatic ductal adenocarcinoma

doi: 10.1101/2024.12.18.628624

Figure Lengend Snippet: A Immunoblotting for ACC & FAS and B measurement of radiolabelled glucose into lipid fraction following knockdown of ACACA or FASN . C Immunoblotting for SCD1 & FADS2 or D quantification of 16:1 isomers measured by liquid chromatography-mass spectrometry following knockdown of SCD or FADS2 . All measurements were performed following 72 h of siRNA transfection. E effect of 48 h GEMC treatment (Panc1 1 µM, MiaPaCa2 100 nM, or vehicle, VEH) on relative cell growth following 72 h knockdown of lipid synthesis enzymes. Cell growth was measured by crystal violet assay and normalised to start of siRNA knockdown (dashed line). Statistical significance was determined by B unpaired t test, or D & E one-way ANOVA with Tukey posthoc correction, * P < 0.05 ** P < 0.01. *** P < 0.001

Article Snippet: Panc1 and MiaPaCa2 cells from ATCC were cultured in high glucose DMEM containing 4.5 g/L glucose and were supplemented with 10% FCS.

Techniques: Western Blot, Knockdown, Liquid Chromatography, Mass Spectrometry, Transfection, Crystal Violet Assay

Journal: bioRxiv

Article Title: Targeting de novo lipogenesis improves gemcitabine efficacy in pancreatic ductal adenocarcinoma

doi: 10.1101/2024.12.18.628624

Figure Lengend Snippet: C De novo lipogenesis measured by the incorporation of radiolabelled glucose into the lipid fraction. Immunoblotting for total and activated key lipid synthesis enzymes in D Panc1 and E MiaPaC2 CON and CombAT cells. F 16:1 fatty acid isomers measured by LC-MS of AMP + -derivatised fatty acids. Measurement of G SCD1 and H FADS2 activity in Panc1 and MiaPaCa2 CON and CombAT cell lines by tracing uniformly stable-labelled palmitic acid into isomeric MUFA. I Analysis of PDAC tumour RNAseq data published by Zhou and colleagues obtained from patients who underwent surgical resection prior to chemotherapy (chemo-naïve) or received GEMC/nab-paclitaxel neoadjuvant treatment (Neoadj.) before tumour resection. J Effect of 72 h knockdown of lipid synthesis enzymes on Panc1 CombAT cell growth. Statistical significance was determined by unpaired t test ( A-I ) or one-way ANOVA with Tukey posthoc comparisons with si NEG only ( J ). * P < 0.05 ** P < 0.01. *** P < 0.001.

Article Snippet: Panc1 and MiaPaCa2 cells from ATCC were cultured in high glucose DMEM containing 4.5 g/L glucose and were supplemented with 10% FCS.

Techniques: Western Blot, Liquid Chromatography with Mass Spectroscopy, Activity Assay, Knockdown